PeronLab@NYU

a cortical circuits & behavior lab

 
 projects

Introduction

Cortical neurons represent sensory and motor features. What circuit mechanisms shape cortical coding? Which neurons drive behavior? Our lab addresses these questions using optical approaches in head fixed mice.

Cellular basis of perception

By combining carefully designed behavioral assays with advanced optical techniques, we hope to determine the minimal subset of neurons whose perturbation can impact behavior.

Circuit mechanisms driving receptive fields

In sensory cortex, neurons with common tuning are more interconnected than neurons with distinct tuning. We are probing how such connectivity enables computations like amplification, pattern completion, and feature detection.

Automated analysis pipelines

Our experiments depend on rapid turnaround of terabyte-scale datasets. We are developing pipelines to process data acquired during calcium imaging and behavioral videography, and to relate neural activity to behavior.

 
 approach

Quantitative behavior

Approaches such as high speed videography and image processing allow precise measurements of the behavioral state of the animal. Tasks are designed so that even subtle changes in the animal's behavior can be detected.

Two-photon calcium imaging

Two-photon microscopy using modern calcium indicators allows us to record the activity of thousands of cortical neurons during behavior. Neurons can be tracked over months, and structural indicators can label cell types.

Mesoscale mapping

A prerequisite to exploring the behavioral roles of neurons is understanding the spatial distribution of functional neural types. We are actively generating mesoscale maps of cortex during behavior, on the 10K-100K neuron scale.

Cellular resolution perturbation

Neurons encoding particular features are typically intermingled with other neurons from which they are genetically inseparable. Approaches like multiphoton ablation and two-photon optogenetics allow for lesioning and activation with cellular precision, making it possible to target intermingled populations.

 
 papers


 

Comprehensive imaging of cortical networks

Peron SP, Chen TW, Svoboda K

2015, Curr. Opinion Neurobiology

 

A cellular resolution map of barrel cortex activity during tactile behavior

Peron SP, Freeman J, Iyer V, Guo C, Svoboda K

2015, Neuron

 

Multiple dynamic representations in the motor cortex during sensorimotor learning

Huber D, Gutnisky DA, Peron SP, O'Connor DH, Wiegert JS, Tian L, Oertner TG, Looger LL, Svoboda K

2012, Nature

 

From cudgel to scalpel: toward precise neural control with optogenetics

Peron SP, Svoboda K

2011, Nature Methods (outlook)

 

Neural activity in barrel cortex underlying vibrissa-based object localization in mice

O'Connor DH, Peron SP, Huber D, Svoboda K

2010, Neuron

 

Complete list of publications, PubMed



 
 people


Simon Peron, PhD

Principal Investigator

Simon earned his PhD with Fabrizio Gabbiani at Baylor College of Medicine, studying single neuron computation in the context of insect vision. He did his postdoctoral work with Karel Svoboda at Janelia Farm, working on mechanisms of cortical processing in the behaving mouse using two-photon microscopy.





Ravi Pancholi

Graduate Student

Ravi joined the lab in 2018 and is studying the neural mechanisms of perception using both naturalistic and synthetic stimuli.



Tina Voelcker

Graduate Student

Tina joined the lab in 2018 and is studying how vibrissal motor cortex encodes movement.



 
 positions


General

We are actively recruiting post-docs and PhD candidates. If you are interested in our science, especially with respect to the projects below, please get in touch!

Perception at cellular resolution

Canonical experiments from the laboratories of Bill Newsome and Ranulfo Romo demonstrated that perturbing small groups of sensory cortical neurons can influence perception. Modern optical techniques open the door to performing cellular resolution loss- and gain-of-function experiments, potentially allowing the field to understand how individual neurons, and not just small patches of cortex, influence perception. We are using a combination of naturalistic and synthetic perception paradigms, in conjunction with multiphoton ablation and two-photon optogenetics, to probe the way in which small groups of neurons impact animal choice and, ultimately, perception.

Circuit mechanisms driving functional selectivity

The famed Canadian neuropsychologist Donald Hebb long ago proposed that neurons that fire together wire together. Recent work has shown that sensory cortical neurons are indeed wired in such a homotypic manner -- that is, neurons with similar sensory tuning are interconnected far more than other neurons. What is the computational function of these enhanced connections? How do they shape neural activity? Are there other mechanisms that contribute to the enhanced feature selectivity these connections are believed to drive? We use two preparations to ask these question: mice performing a vibrissal object-localization task, and mice performing a synthetic perception task where the stimulus is exclusively optogenetic.



 
 contact


Peron Lab

Center for Neural Science

New York University

4 Washington Place, Rm. 809

New York, NY 10003